Figure 5.
Depletion of p21CIP1/WAF1 interferes with miR-34–induced inhibition of cancer cell proliferation. miRNA mimics and small interfering RNAs were transiently transfected into isogenic RKO cells as shown in the graph. Protein expression was verified by (a) Western analysis, and proliferation was assessed by (b) AlamarBlue. Proliferation data are normalized to cells transfected with negative control (100%). Averages and standard deviations are shown. P values were derived from two-tailed Student's t tests. HDAC1, histone deacetylase 1.