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. 2013 Aug 20;21(9):1653–1660. doi: 10.1038/mt.2013.150

Figure 3.

Figure 3

GJ071 and GJ072 induced ATMs1981 foci formation in A-T cells with a TAA mutation; full-length ATM protein levels were abrogated in A-T cells with a TGA mutation. A-T cells were treated with each compound for 4 days prior to testing for read-through effects. Wild-type cells were used as positive controls. (a) RTC-induced ATM1981 foci in AT187LA cells with a homozygous TAA mutation. The experiments were repeated multiple times; a representative experiment is used here to show ATMs1981 foci formation after 2-Gy radiation. The formation of γ-H2AX foci was included as a control for effective radiation damage; (b) Quantification of Figure 3a for IRIF-positive cells percentage; >120 cells were counted for each sample; (c) RTC-induced ATM protein in AT153LA cells with TGA mutation measured by ATM-ELISA. *P ≤ 0.05, compared with nontreated A-T cells. A-T, ataxia telangiectasia; RTC, read-through compounds.