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Sphk-1 and CD14 mRNA expression in LPS-treated Kupffer cells. Total RNA extracted from Kupffer cells treated with LPS was examined for (A) Sphk-1 and (B) CD14 mRNA expression by Q-PCR and presented as fold change over GAPDH. Data are presented as the mean ± SEM and compared by Student’s t-test. *P<0.05 vs. control. Sphk-1, sphingosine kinase-1; LPS, lipopolysaccharide; Q-PCR, quantitative PCR.
