Figure 2.

Prophylactic treatment with NPD1 reduces Th1 and Th17 cell responses in corneas of HSV-1–infected animals. C57BL/6 mice infected with 2 × 10⁴ PFU of HSV-1 RE were given NPD1 topically twice daily starting from 1 day before infection until day 10 pi. (A) Representative plots show percentage of CD4⁺ T cells producing IFN-γ or IL-17 following stimulation with CD3/CD28 in the corneas of control and NPD1-treated animals. Plots shown were gated on CD4⁺ T cells. (B, C) Average number of CD4 T cells producing (B) IFN-γ and (C) IL-17 in the cornea at day 15 are shown. The level of significance was determined by Student's t-test (unpaired). Error bars represent mean ± SEM, n = 4 corneas per group. Experiments were repeated at least two times *P ≤ 0.05. **P ≤ 0.01.