Figure 6.

NPD1 treatment reduces the levels of cytokine and chemokines in corneas of HSV-1–infected animals. C57BL6 mice infected with 2 × 10⁴ PFU of HSV-1 RE were given NPD1 topically twice daily starting from day 6 until day 12. Mice were killed at day 15 pi, and levels of cytokines and chemokines were measured in three different pooled corneal samples, each consisting of five corneas/group in control and NPD1-treated animals by sandwich ELISA or QPCR. (A) IL-6 protein levels were quantified using sandwich ELISA. (B) CXCL1 levels were quantified using sandwich ELISA. (C, D) qPCR was used to measure the expression of (C) CXCL-10 and (D) CCL-20 in corneal samples obtained from control and NPD1-treated animals. (E) IL-10 protein levels were measured using sandwich ELISA. The level of significance was determined by Student's t-test (unpaired). Error bars represent mean ± SEM, n = 3 samples per group. *P ≤ 0.05. **P ≤ 0.01.