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(A) Nitric oxide production in cocaine-treated astroglial cells. The cells were seeded in 96-well plates with complete RPMI-1640 media lacking phenol red, containing 10% FBS and treated with 2, 3 and 4 mM cocaine for 1 h. Nitric oxide (NO) was detected with Griess reagent. Data are presented as means ± SEM (n=12, *P>0.05, insignificant in comparison to the control). (B) Standard curve of sodium nitrite (25–400 μM).
