Table II.
Circulating monocytes, peripheral blood CD34+ cells, their apoptotic rate and CFU-GMs in a series of NS, NS/MPD and JMML patients.
| Clinical parameters | Controls | NS | NS/MPD | JMML |
|---|---|---|---|---|
| Monocytes (/μl) | 600 (200–900) | 695 (300–1,720) | 2,550 (2,500–5,520) | 1,600 (1,070–7,600) |
| CD34+ (/μl) | 5.2 (1.8–23.1) | 4.9 (1.3–17.5) | 205.7 (58–1,374) | 109.8 (44–232) |
| Annexin V+/CD34+ (%) | 17.6 (2.8–49.6) | 8.6 (0.0–27.7) | 1.4 (0.2–2.4) | 2.1 (0.4–12.1) |
| GM-CSF (20 ng/ml) | 9 (0–26) | 4 (0–34) | 36 (0–84) | 46 (18–258) |
| GM-CSF (10 ng/ml) | 5 (0–18) | 2 (0–30) | 38 (0–76) | 40 (10–244) |
| GM-CSF (5 ng/ml) | 3 (0–18) | 1 (0–24) | 38 (0–50) | 42 (6–262) |
| GM-CSF (1 ng/ml) | 1 (0–16) | 1 (0–23) | 34 (0–62) | 32 (6–264) |
| GM-CSF (0.1 ng/ml) | 0 (0–14) | 0 (0–8) | 26 (0–36) | 30 (2–240) |
| Unstimulated | 0 (0–0) | 0 (0–4) | 4 (0–32) | 18 (0–84) |
NS, Noonan syndrome patients (n=24); NS/MPD, Noonan syndrome patients with myeloproliferative evolution (n=3); JMML, myelomonocytic leukemia patients (n=5). CFU-GMs from 2×105 circulating mononuclear cells were cultured in the presence of decreasing GM-CSF concentrations and without GM-CSF (unstimulated). Data are provided as mean value (range).