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. 2013 Sep 18;8(9):e75227. doi: 10.1371/journal.pone.0075227

Figure 2. Uptake of monocyte-derived exosomes by MSCs.

Figure 2

PKH67-labelled (green) monocyte exosomes or PKH67 control were added to MSCs in culture and the uptake was examined after 72 h using flow cytometry (a-d) and fluorescence microscopy (e-h). (a) Shows MSCs cultured with PKH67 control, (b) MSCs cultured with PKH67-labelled exosomes, (c) the percentage of MSCs positive for the PKH67 stain after 72 h and (d) the mean fluorescence intensity of the MSCs after co-culture with the green exosomes or control (n = 4, *p<0.05). (e-h) Fluorescence micrograph of MSCs (blue nucleus stained with DAPI). (e) MSCs co-cultured with control PKH67. (f-h) MSCs co-cultured with exosomes (green) show that some cells are positive for the exosomes while others are negative. (g) The intensity of the staining varies between cells.