Table 2. Bacterial strains and plasmids used in this study.
| Designation | Relevant Feature | Ref. or Source |
|---|---|---|
| Strain | ||
| M. xanthus | ||
| DK1622 | Wild type | |
| DK10410 | DK1622, ΔpilA, missing PilA | [41] |
| MXH2265 | DK1622, ΔaglZ, deficient in A-motility | [33] |
| SW2022 | DK1622, ΔaglZ, ΔpilA | [14] |
| YL6101 | DK1622, ΔpilA, containing pTZG-1 | This study |
| YL6102 | DK1622, ΔpilA, containing pTZG-2 | This study |
| YL6103 | DK1622, ΔpilA, containing pTZG-3 | This study |
| YL6104 | DK1622, ΔpilA, containing pTZG-4 | This study |
| YL6105 | DK1622, ΔpilA, containing pZJY41 | This study |
| YL6106 | DK1622, ΔpilA, containing pTZG-5 | This study |
| YL6111 | DK1622, ΔaglZ, ΔpilA, containing pTZG-1 | This study |
| YL6112 | DK1622, ΔaglZ, ΔpilA, containing pTZG-2 | This study |
| YL6113 | DK1622, ΔaglZ, ΔpilA, containing pTZG-3 | This study |
| YL6114 | DK1622, ΔaglZ, ΔpilA, containing pTZG-4 | This study |
| YL6115 | DK1622, ΔaglZ, ΔpilA, containing pZJY41 | This study |
| YL6116 | DK1622, ΔaglZ, ΔpilA, containing pTZG-5 | This study |
| S . aurantiaca | ||
| DSM17044 | Type strain for S . aurantiaca , ATCC 25190 | [24] |
| E. coli | ||
| DH5α | Host for cloning | [61] |
| Plasmid | ||
| pZJY41 | Shuttle vector in E. coli-M. xanthus; Kanr Ampr | [32] |
| pTZG-1 | PSPMx and pilA Sa1 fusion fragment in pZJY41* | This study |
| pTZG-2 | PSPMx and pilA Sa2 fusion fragment in pZJY41 | This study |
| pTZG-3 | PSPMx and pilA Sa3 fusion fragment in pZJY41 | This study |
| pTZG-4 | PSPMx and pilA Sa4 fusion fragment in pZJY41 | This study |
| pTZG-5 | PSPMx and pilA Mx fusion fragment in pZJY41* | This study |
*
pilA Mx stands for pilA in M. xanthus DK1622, PSPMx stands for promoter and signal peptide sequence of pilA gene in M. xanthus DK1622, and pilA Sa stands for pilA in S . aurantiaca DSM17044.