Figure 5. Long-term OVX increased Ca²⁺ currents and Ca²⁺ transients, but had no effect on EC-coupling gain.

A. Voltage clamp protocol shown at top. Representative Ca²⁺ transients and Ca²⁺ currents recorded in myocytes from sham and OVX mice. B,C. Mean Ca²⁺ current densities and Ca²⁺ transients were increased by long-term OVX. D. The gain of SR Ca²⁺ release was similar in both groups. E. Diastolic Ca²⁺ levels were similar in the two groups at all voltages tested. F. The time course of inactivation (tau) for Ca²⁺ currents activated by a test step to 0 mV was similar in sham and OVX cells. G. The normalized integral of the Ca²⁺ current activated by a test step to 0 mV was larger in OVX myocytes compared to sham controls (n=14 sham and 16 OVX myocytes; *p<0.05)..