Figure 1. The G32E substitution prevents ligand-induced transcription activity of sPPARD.

(A) Glycine 32 in the A/B domain of sPPARD is conserved across mammals as described in [9]. The asterisk and red line indicates the positions of glycine 32 and lysines 16–18. (B) PK-15 cells were transfected with pcDNA4A-His, wild-type sPPARD, and G32E mutant expression vectors along with PPREx3-tk-Luc and pCX-nLacZ reporter vectors. Cells were incubated overnight with DMSO or GW0742 (5 µmol/L). The ratio of firefly luciferase to β-galactosidase activity was defined as relative luciferase activity. Data represent -fold increase (mean ±S.D. of triplicate experiments) relative to empty vector-transfected and ligand untreated cells. * P<0.05 indicates significant difference between wild-type and G32E mutant sPPARD in the presence of ligand.