Figure 2. The G32E substitution stimulates CRM1-mediated nuclear export of sPPARD.

PK-15 cells were transfected with pEGFP-C1-wild-type sPPARD (A), and -G32E mutant (B). G32E mutant was treated by leptomycin B (50 nM, 1 h) that blocked CRM1-dependent nuclear export (C). sPPARD subcellular localization was analyzed by GFP fluorescence at 24 hour post-transfection. Cell nuclei were counterstained with DAPI.