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. 2013 Sep 18;8(9):e75925. doi: 10.1371/journal.pone.0075925

Figure 3. The G32E substitution does not alter the binding affinity of sPPARD with ligand.

Figure 3

The surface plasmon resonance analysis was performed, and the sensorgrams were obtained from injection of GW0742 at 5 concentrations over surface-immobilized wild-type sPPARD (G32, A) or G32E mutant (E32, B). GW0742 were injected for 60 s, and dissociation was monitored for more than 120 s. The concentrations (µmol/L) are shown next to the arrows. For the kinetic analysis, wild-type sPPARD and G32E mutants of five reference-subtracted sensorgram signals were globally fitted into a 1∶1 interaction model [19], giving an association rate constant (Ka), and a dissociation rate constant (Kd). The equilibrium dissociation constant (KD) was calculated by the ratio of rate constant (Kd/Ka).