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. 2013 Sep 18;8(9):e75723. doi: 10.1371/journal.pone.0075723

Figure 3. Sumo mRNA is not strongly affected by ER stress in mammalian cells.

Figure 3

A. Conservation of the Ire1 site and surrounding region in sumo transcripts across species. The stem loop is indicated above the sequences and the region aligning with the loop from D. melanogaster sumo is shown in red. Deviations from the D. melanogaster sequence in the stem loop region are shown in blue. While most species have a conserved loop sequence, perfect basepairing in the stem is present only in the fly and mouse sequences. B-C. We either mock-treated (control) or used siRNA to deplete Ire1α from MC3T3-E1 mouse osteoblastic fibroblasts (panel B) or Hek293 human kidney cells (panel C). We then compared RNA levels in the presence and absence of DTT (2 mM, 4.5 hrs), by qPCR. Blos1, a RIDD target in mouse and humans, is shown as a control. Except for Blos1, the differences in mRNA levels between control and Ire1 siRNA-treated cells were not statistically significant. Shown are the averages and SDs for 2–3 independent experiments.