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We used RNAi to deplete S2 cells of Perk. A. Western blot showing the levels of phosphorylated and total eIF2α. B. Agarose gel showing the relative levels of unspliced and spliced Xbp1, amplified by reverse-transcription-PCR using primers surrounding the splice site. C. Relative mRNA levels in ER stress treated vs untreated cells, determined by qPCR. Panels A-B show representative data, panel C is the average and SD of 3 independent experiments.
