Table 1. Quantitative SIMS imaging analysis of boron concentrations in tumor cells from B16 melanoma bearing mice.
| Treatment time (hr)a | Boron conc. (µg/g wet weight) | SIMS imaging fieldsb |
| 1.0 | 28±7c | 5 |
| 2.5 | 14±5d | 7 |
| 4.0 | 17±6d | 7 |
Four mice in each treatment received 24 mg boron/kg b.w. cis-ABCPC via injection. After 1, 2.5, and 4 hr. post injection, the mice were euthanized and samples of tumor i.p. were frozen for SIMS studies. The boron concentrations from SIMS images, as shown in Fig. 2, are expressed in µg/g wet wt. (mean ± SD).
In the 1 hr treatment group, observations represent 31 regions of interest (ROIs) in 5 SIMS imaging fields. A region of interest within a SIMS imaging field is defined as a clump of 10-15 individual cells taken together for quantification of SIMS images and, therefore, representing a large sampling of the imaging data. In 2.5 hr treatment, observations represent 47 ROIs in 7 SIMS imaging fields. In 4 hr treatment, observations represent 51 ROIs in 7 SIMS imaging fields. The superscript “c” denotes the significant difference (P<0.05) in boron concentrations from treatments designated with superscript “d”.