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Representative example of a single plane of a deconvolved image of a single cell stably expressing AQP3-EGFP and Orange-AQP4 10 minutes following release from a 19°C temperature block and inserts of the marked areas: green arrows point to AQP3-EGFP containing TGN and post-Golgi carriers, red arrows point to Orange-AQP4 containing TGN and post-Golgi carriers. Scale bars are: 5 μm (overlay and single channel); inserts: 0.5 μm.
