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. 2013 Sep 18;8(9):e74174. doi: 10.1371/journal.pone.0074174

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© 2013 Leung et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Sweat gland LRCs are attached to the basement membrane positive for β4 integrin (red) and are (B) found in the basal layer co-localizing with K14. (C,D) Sweat gland LRCs do not express lumenal layer markers K8 and K18, respectively. (E) Within the basal layer, LRCs co-localize with myoepithelial cell marker p63, inset denotes p63 single channel, arrows and (F) with myoepithelial cell marker smooth muscle actin (SMA), arrows. (G,H) Whole mount staining of SGs with laminin with and without DAPI. Arrows denote co-localization of markers with H2BGFP marked LRCs. Abbreviations: LRCs, label-retaining cells; H2BGFP, histone 2B conjugated with green fluorescent protein; SMA, smooth muscle actin; K, keratin.