Figure 2. Effect of anti-TLR4 pAb on secreted embryonic alkaline phosphatase (SEAP) release in MPLA and pro-oxidant-stimulated HEK-Blue mTLR4 cells.

Cells were preincubated with anti-TLR4 pAb (5 µg/ml) for 2 h followed by stimulation overnight with either MPLA (57 nM) or PPC (1 µM). Preincubation with rat polyclonal IgG (10 µg/ml) was used as control. SEAP release in the supernatant was determined using Quanti-Blue with the absorbance read at 650 nm. The data represent 3 independent experiments. *p≤0.001 vs treatments with MPLA or PPC preincubated with anti-TLR4.