Table 4. HPLC based quantification of different organic acids from control and TU treated pods.
Treatments | Phophoenol pyruvate (μg g-1) | Pyruvate (μg g-1) | Malate (μg g-1) | |
2 d | Control | 29±0.4 | 22±0.6 | 494±40 |
Thiourea | 33*±0.6 | 22±0.5 | 499±18 | |
5 d | Control | 41±1.0 | 11±0.2 | 331±21 |
Thiourea | 50*±0.6 | 25*±2.0 | 468*±22 | |
15 d | Control | 29±1.5 | 7±0.4 | Not detectable |
Thiourea | 50±2.3 | 10*±0.6 | 466*±13 |
The values represent mean ± SD of three independent biological replicates. The significance of mean difference (P<0.05) was evaluated on the basis of student t-test and marked with asterisk (*).