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. 2013 Sep 18;8(9):e73981. doi: 10.1371/journal.pone.0073981

Figure 4. siRNA-mediated downregulation of CD300a increases monocyte transendothelial migration.

Figure 4

A. FACS analysis of cell surface CD300a expression in monocytes transfected with control or CD300a siRNAs, respectively, employing mouse monoclonal anti-CD300a and appropriately labelled secondary antibodies. Control cells were incubated without primary and secondary (control 1) or only with secondary antibodies (control 2). B. Freshly isolated monocytes from human buffy coats were transfected with control or CD300a siRNAs, respectively, followed by transendothelial migration analysis. The number of transmigrated cells is given on the y-axis. Three independent experiments were performed and results are presented as average +/− SEM. Statistical significance was evaluated using student's t-test. * P<0.05.