Figure 6. Anti-CD300a antibody treatment alters the morphology of human monocytes.

Freshly isolated human monocytes were treated with anti-CD300a IgGs or isotype matched control IgGs (Anti-c-myc) and then seeded on cover slips and incubated at 37°C for an additional 30 min. Cells were then fixed and stained with TRITC-phalloidin and anti-CD300a followed by appropriate secondary antibodies. Scale bars 10 μm.