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. 2013 Sep 18;8(9):e74608. doi: 10.1371/journal.pone.0074608

Figure 4. Quantitative results of the ratio of the PKCα (top panel) and CPI-17 (bottom panel) at the cells periphery to the total protein (peripheral+cytosolic) in the circular and longitudinal layers of the antrum and fundus.

Figure 4

The ratio of PKCα in the relaxed condition (PSS) in both layers of antrum and fundus is significantly greater than 0.5, indicating a preferential distribution of PKCα near the plasma membrane in the relaxed condition. This ratio does not change significantly with different stimulation treatments, suggesting that PKCα maintains a primarily peripheral distribution in the cell at all times. (n = 5). The CPI-17 ratio in the relaxed condition (PSS) in both layers of antrum and fundus is not significantly different than 0.5, indicating a uniform distribution of CPI-17 throughout the cells. Three minutes of CCh stimulation does not change the ratio of CPI-17 except in the fundus circular layer. Both CCh (30′) and PDBu (30′) treatments cause a significantly redistribution of CPI-17 to the cell periphery near the plasma membrane (ratios are significantly greater than 0.5). * p<0.05, **p<0.01 (n = 3).