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. 2013 Sep 18;8(9):e74523. doi: 10.1371/journal.pone.0074523

Figure 1. CLIC1 ion channel activity from Tip-Dip bilayer experiments.

Figure 1

Current recordings from -80 to +80 mV, 20 mV interval, are shown in (A) for wild type CLIC1 (left), K37A (center) and R29A (right) CLIC1 protein. The upper panel of Figure 1B depicts the single-channel current-voltage data for WT (□) and K37A (○) CLIC1 proteins. The average single-channel conductance differs between WT and K37A, calculated as 30.1 ± 0.2 and 42.4 ± 0.2 pS, respectively (n = 5, p < 0.001). In contrast, the channel open probability of the K37A mutation is very similar to the WT (Figure 1B, lower panel). The current/voltage relationships for both WT (□) and R29A CLIC1 (○) are shown in (C). The single-channel conductance (upper panel), is 30.1 ± 0.2 and 29 ± 0.2 pS for WT and R29A, respectively. The average open probability for WT (□) and R29A mutated protein (○) is shown in the lower panel.