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. 2013 Sep 18;8(9):e74523. doi: 10.1371/journal.pone.0074523

Figure 2. Endogenous CLIC1 is not expressed on the plasma membrane of untransfected HEK cells.

Figure 2

(A) Family of currents for an untransfected HEK293 cell at varying applied voltages from -60 to +60 mV with 20 mV increment. Top panel: whole cell current in resting conditions; middle panel: after IAA94 perfusion; lower panel: IAA94-sensitive (CLIC1-mediated) current obtained from subtraction. (B) i/V curve of an IAA94-sensitive current in WT CLIC1 transfected HEK293 cell (□), and in an untransfected HEK293 cell (○). (C) Plot of the average of the IAA94-sensitive current as a percentage of the control current of untransfected HEK cells (n=5). Data are shown as mean ± SEM. CLIC1-mediated current is completely absent in untransfected HEK cells.