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. 2013 Sep 18;8(9):e74523. doi: 10.1371/journal.pone.0074523

Figure 7. Whole-cell current in R29A transfected HEK cells.

Figure 7

Family of whole-currents for WT (A) and R29A (B) transfected HEK cells (top panels). Voltage steps lasting 800 ms from holding potential of -30 mV to membrane potential of -80 to +100 mV (20 mV step increment). The middle panels depict the whole-cell currents after perfusion of 50 µM of IAA94. The bottom panels represent the IAA94-sensitive currents obtained by subtraction of the middle panel current from the upper panel current. Note that the IAA94-sensitive currents are plotted on a different scale with the scale bars on the right hand side of the figure. (C) an example of current/voltage relationship of the IAA94-sensitive current from a WT (□) and a R29A (○) transfected HEK cell. (D) Averaged G/V plots from IAA94-sensitive current of WT (□) and R29A mutant (○), from 5 independent experiments.