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. 2013 Sep 18;8(9):e74538. doi: 10.1371/journal.pone.0074538

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© 2013 Wang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The five selected compounds inhibited SPS formation (magnification, 200×). (B) Niclosamide inhibited MCF7 SPS formation. The SP of MCF7 was treated with 3 µM or 30 µM niclosamide for 48 h (magnification, 50×). Images of MCF7 SPS culture were analyzed using the Image-Pro Plus 6.0 software. (C) Niclosamide decreased the fraction of MCF7 SP. Treatment with niclosamide decreased the SP fraction in MCF7 and MDA-MB-231 cells. (D) MCF7 SPS were treated with niclosamide (3 µM) for 72 h. Apoptosis was evaluated using flow cytometry.