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. 2013 Sep 18;8(9):e74232. doi: 10.1371/journal.pone.0074232

Figure 5. Effect of CK2 inhibition on Phe508delCFTR synthesis.

Figure 5

BHK cells expressing human CFTR wild type (A), T1471A (B) and Phe508del (C) were treated for 2 h with CHX to stop protein synthesis (compare first and second lanes). Cells were washed with PBS and incubated for the indicated time with either CK2 specific inhibitor CX4945 or its vehicle. Proteins from each sample were separated by gel electrophoresis and blotted on nitrocellulose membranes (see Materials and Methods). Blots were developed for the indicated antibodies. In (D) quantitation of Phe508delCFTR band B expression after the indicated treatment is shown. The figure is representative of experiments performed in triplicate. Due to the low expression level, Phe508delCFTR signal was obtained by overexposed conditions.