Figure 1. Effect of physical size difference on crayfish agonistic bouts.

A, Winning rate of larger crayfish during agonistic bouts in pairings with smaller crayfish. The percentages of outcomes of agonistic bouts of larger crayfish are plotted as wins (black), losses (light grey) and draws (dark grey). Left: pairings between naive large (NL) and naive small (NS) animals, Middle: pairings between dominant large (DL) and dominant small (DS) animals, and Right: pairings between subordinate large (SL) and subordinate small (SS) animals. B, The time taken for the dominant-subordinate relationship to form. Box plots show median (solid black line), interquartile range (box length), and minimum and maximum values (error bars). Open circles indicate pairs that did not show clear dominant-subordinate relationship after 45 mins of pairings. C, The number of fights between pairings during the first 30 min agonistic bouts. D, The average duration of individual fights of pairings during the first 30 min of agonistic bouts. In C and D, the number of fights and the average duration of individual fights in subordinate pairings are separately plotted whether the dominant-subordinate relationship was determined or not. Asterisks in C and D indicate that the number and duration of responses differed significantly (Mann-Whitney rank sum test, *p<0.05, **p<0.01).