Skip to main content
. 2013 Sep 18;8(9):e74069. doi: 10.1371/journal.pone.0074069

Figure 2. Production and transcription of the LT toxin depended on CRP is higher at early hours of the bacteria growth.

Figure 2

E2863 wt and E2863ΔCRP were growth in LBK media and samples were taken at 3, 5 hours and from overnight cultures. GM1 ELISA was used to measure the total production and secretion of LT toxin. Expression of the toxin was quantified in eltAB gene for LT toxin by q-PCR. Bars represent the mean and error bars represent the standard deviations of n = 9 (ELISA) and n = 3 (q-PCR). A Student t test was used to calculate P values, *P<0.05, **P<0.01. A) Total production. B) Percentage of secretion C) Number of transcripts of eltAB gene.