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. 2013 Sep 18;8(9):e74069. doi: 10.1371/journal.pone.0074069

Figure 5. Intracellular levels of cAMP are increased in a CRP mutant.

Figure 5

cAMP was measured by cAMP ELISA assay in the pellet and supernatant in E2863wt and E2863ΔCRP strains. The strains were grown in LBK media (A) and in LBK media adjusted at different pH (B and C) for 3 hours. Intracellular cAMP is defined as the cAMP detected in the pellet and extracellular cAMP as the cAMP present in the supernatant. Bars represent the mean and error bars represent the standard deviations of 4 independent experiments. A Student t test was used to calculate P values *P<0.05, **P<0.01, ***p<0.001. A) cAMP in LBK media, B) intracellular cAMP at different pH ranges, C) extracellular cAMP at different pH ranges.