Table 1. Bacterial strains, mutants and recombinant plasmids used.

Strain, mutant, and/or plasmid	T3SS Description	Replicates in Epithelial cells	Reference
PAO1	Wild-type P. aeruginosa. Expresses ExoS, ExoT, ExoY	+	[13,29]
PAO1exsA::Ω	Lacks T3S transcriptional activator	-	[29,48]
(exsA mutant)	No T3S expression
PAO1ΔpopB	Lacks T3S translocon	+	[13,29]
(popB mutant)	Encodes ExoS, ExoT, ExoY
PAO1ΔexoSTY	No known T3S effectors	-	[13,29]
PAO1ΔexoSTY + pUCPexoS	Complementation with plasmid-expressed ExoS	+	[16,49]
PAO1ΔexoSTY + pUCPexoS (E381D)	Complementation with plasmid-expressed ExoS without ADPr activity	-	[16,49]
PAO1ΔexoSTY + pUCP18	Plasmid control	-	[16]
pSMC2	Plasmid encoding constitutively-expressed green fluorescent protein (GFP)	NA	[31]
pJNE05	Plasmid encoding gfp fused the ExsA-dependent promoter of exoS	NA	[33]
p67T1	Plasmid encoding constitutively expressed dTomato	NA	[32]