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. 2013 Aug 2;154(10):3877–3887. doi: 10.1210/en.2013-1341

Figure 1.

Figure 1.

GnRHR expression in the pituitary and extrapituitary tissues. A, GnRHR expression in the pituitary and selected extrapituitary tissues. Top panel, GnRHR expression was tested by RT-PCR using primers F1–R1 in exons 2 and 3 (see Materials and Methods). Bottom panel, Control RT-PCR for β-actin. The tissues labeled with an asterisk indicate presence of GnRHR expression. BL, bladder; BR, brain; CO, colon; CE, cerebellum; HT, heart; KI, kidney; LI, liver; LU, lung; MS, muscle; NC, negative control; OV, ovary; PI, pituitary; SI, small intestine; SP, spleen; TE, testis; UT, uterus. B, GnRHR mRNA expression within the mouse ovary. Primers used were 2.3F-2.3R in exons 2 and 3 (see Materials and Methods). The top band is the specific band, and the lower band (labeled with a bold arrow) is a nonspecific band also seen in the negative control. CC, cumulus cells; GC, granulosa cells; NC, negative control; OO, oocyte; OV, ovary.