Figure 1.

BRET² saturation curves indicating heterodimerization of the LHR and FSHR. HEK293 cells were transiently transfected with the indicated pairs of fusion constructs under conditions in which the Rluc fusion protein was held constant and increasing amounts of the GFP² fusion protein were expressed. Data shown are the corrected BRET² signals as a function of GFP²/Rluc expression. Within a given experiment, the plasmid concentrations were adjusted so that all Rluc fusion proteins in the absence of GFP² fusion proteins were expressed similarly. Data shown are from one experiment representative of 4 independent experiments.