Table 1.
Primers for qRT-PCR
| Transcript | Accession No. | Direction | Nucleotide Sequence (5′ Position) |
|---|---|---|---|
| Ppib | NM_011149.2 | F | 5′-TGGAGAGCACCAAGACAGACA (642) |
| R | 5′-TGCCGGAGTCGACAATGAT (707) | ||
| Kdm5c | NM_013668.3 | F | 5′-GAGGCCCAGACAAGAGTGAAA (557) |
| R | 5′-TTGGGAATCTTTAAGGATGAGCC (642) | ||
| Kdm6a | NM_009483.1 | F | 5′-CGGGCGGACAAAAGAAGAAC (4299) |
| R | 5′-CATAGACTTGCATCAGATCCTCC (4501) | ||
| Eif2s3x | NM_012010.3 | F | 5′-GGTGAGGGTGGAGTGACTCT (28) |
| R | 5′-TTCCCATGAGCTACGTGACCA (179) | ||
| Ddx3x | NM_010028.3 | F | 5′-CAGAGTGGAGGAAGTACAGCA (170) |
| R | 5′-TCACCCCGTGATCCAAAACTG (321) | ||
| Gh | NM_008117.2 | F | 5′-AGGCCCAGCAGAGAACCGACA (333) |
| R | 5′-ACGGTCCGAGGTGCCGAACA (460) |
Oligonucleotide sequence of primers used to amplify the mRNA transcripts of the genes Kdm5c (lysine-specific demethylase 5C), Kdm6a (lysine specific demethylase 6A), Eif2s3x (eukaryotic translation initiation factor 2, subunit 3, structural gene X-linked), Ddx3x (DEAD/H box polypeptide 3, X-linked), Gh (growth hormone), and Ppib (cyclophilin B). Accession numbers are from the National Center for Biotechnology Information Reference Sequence database. Forward (F) and reverse (R) primers anneal to cDNA antisense and sense strands, respectively. Numbers in parentheses indicate the primers' 5′-nucleotide position on the reference sequence.