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. 2013 Jul 11;154(10):3753–3763. doi: 10.1210/en.2013-1201

Figure 4.

Figure 4.

Amphioxus IGFBP Has Transcriptional Activation Activity. A, The N domain of amphioxus IGFBP was fused to the Gal4-DBD and introduced into HEK 293T with a Gal4 reporter plasmid. The human IGFBP-5 N domain and the human IGFBP-1 N domain were fused to the Gal4-DNA-binding domain (DBD) and used as positive and negative controls. The expression of the indicated Gal4 fusion proteins was verified by immunoblot, and this is shown in the lower panel. B, The indicated amphioxus IGFBP N domain mutants were fused to the Gal4-DBD and their transcriptional activities were determined and are expressed as relative values of the control group. Transfection efficiency was normalized by Renilla luciferase activity. Values are means ± SEM; n = 3. *, P < .05 compared with the pBind group. The expression of the indicated Gal4 fusion proteins was verified by immunoblot and this is shown in the lower panel. MW, molecular weight.