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. 2013 Jul 11;154(10):3753–3763. doi: 10.1210/en.2013-1201

Figure 5.

Figure 5.

Amphioxus IGFBP Has NLS-Dependent Biological Actions Similar to IGFBP-5 but Different from IGFBP-2. A, Classification of phenotypes caused by forced expression of various IGFBPs. 1- to 2-cell stage zebrafish embryos were injected with capped mRNA encoding human IGFBP-2, human IGFBP-5, and amphioxus IGFBP or GFP. They were raised to 24 hours postfertilization (hpf). Lateral views are shown. Scale bar, 0.5 mm. B, Quantitative results. Embryos described in panel A were scored accordingly and the percentage in each category is shown. Total embryo number is shown on the top of each column. C, Nuclear localization is required. Embryos injected with amphioxus IGFBP, its ΔNLS1+ΔNLS2 mutant, or GFP mRNA were raised to 24 hpf and scored and are presented as described above.