Figure 2.

Comparison of changes in the transcriptome following Rv0805 overexpression and in the Δcrp mutant A. Comparison of Rv0805 overexpression gene set and the CRP^Mt regulon.^20,22 Fold regulation of 160 genes from the two data sets were compared on a scatter plot. Pearson's correlation analysis was performed using Graphpad Prism and was found not to be statistically significant (p-value 0.49). B. Comparison of cellular pathways affected by Rv0805 overexpression (outer donut) and CRP^Mt deletion (inner donut). Genes with altered expression levels (>±2 fold) were assigned functional categories according to Tuberculist (www.tuberculist.epfl.ch) annotation. Numbers represent percentage of total genes altered. C. Comparison of genes altered by Rv0805 overexpression and CRP^Mt deletion. Genes dysregulated upon Rv0805 overexpression (upper panel) do not show significant alteration in expression levels in the CRP^Mt knockout and vice-versa (lower panel). Red boxes indicate up-regulation and blue boxes indicate down-regulation compared to appropriate reference strains. Numbers indicate fold change in expression level as obtained from microarray analyses. ‘-’ indicates no change in expression levels (<2.0 fold).