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. 2013 Sep;93(5):492–500. doi: 10.1016/j.tube.2013.05.004

Figure 3.

Figure 3

A. Role of the C-terminus of Rv0805 in gene expression changes. Overexpression of Rv0805N97A and Rv0805(1-278)N97A. Immunoblot for lysates of M. tuberculosis harboring empty vector (control) or plasmid for overexpression of Rv0805, Rv0805N97A or Rv0805(1-278)N97A. Polyclonal antibodies against Rv0805 or a C-terminus specific monoclonal antibody (1G5B5) were used to distinguish between the full length and C-terminally truncated proteins based on differential mobility and loss of immunoreactivity respectively. B. Intracellular cAMP levels in M. tuberculosis overexpressing Rv0805, Rv0805N97A or Rv0805(1-278)N97A. Mean ± SEM of at least two biological replicates are plotted. (n.s. indicates that the two groups compared are statistically not significant (p-value > 0.05)) C. Heat map showing expression levels of genes in M. tuberculosis-Rv0805 compared with a strain harboring empty vector (control) or overexpressing Rv0805N97A. Two distinct gene sets (A and B) are clearly discernible. D. Genes showing altered regulation in the Rv0805 overexpression strain compared to M. tuberculosis harboring empty vector (control) or overexpressing Rv0805N97A or Rv0805(1-278)N97A. Presence of the C-terminus of Rv0805 is essential for manifestation of catalysis-independent gene expression changes. Red boxes indicate up-regulation and blue boxes indicate down-regulation in M. tuberculosis-Rv0805 compared to the indicated strains. Numbers indicate fold change in expression level as obtained from microarray analyses. ‘-’ indicates no change in expression levels (<2.0 fold).