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. 2013 Sep;93(5):482–491. doi: 10.1016/j.tube.2013.05.005

Figure 1.

Figure 1

Co-transcription of sigG, Rv0181c and Rv0180c but not lprO. (A) Schematic representation of M. tuberculosis genomic region containing Rv0182 (sigG)-Rv0179c (lprO). Includes positions of primers used in RT-PCR. (B) RT-PCR showing co-transcription of sigG operon. Transcript was detected using primer sets 1, 3 and 4 but not 2 and 5 indicating sigG transcript terminates within lprO between primer sets 1 and 2 and that lprO transcription starts within Rv0180c. + shows RT positive; − shows RT negative control; D shows genomic DNA PCR control; sizes of DNA markers are indicated.