Fig. 2.

N-type cells stained positive for a neuronal lineage marker whereas S-type cells stained positive for a non-neuronal lineage marker. Confocal micrographs showing β-tubulin III (green), vimentin (red) and EthD-1 (blue). Cells were induced to differentiate by the addition of 1 μM 9cRA for 7 days. Proliferating (A) SH-SY5Y, (B) N-type and (C) S-type cells. Differentiated (D) SH-SY5Y and (E) N-type cells. Vimentin in neurite extension; arrow. (F) Differentiated S-type cells. Scale bars represent 50 μm. The parental SH-SY5Y cell line was comparable to N-type cells.