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. 2012 Aug 30;2(2):321–331. doi: 10.1016/j.celrep.2012.06.015

Figure 2.

Figure 2

Transgenic Rescue of lat-1 Mutants Uncover Different Domain Requirements of the LAT-1 Receptor

(A–C) LAT-1 receptor domain modifications exhibit different capacities to rescue lethality (circles) and fertility (boxes) of lat-1(ok1465) mutants. Note that the increased number of adult survivors in some transgenic lines is mainly due to increased numbers of eggs laid, whereas these constructs have little effect on the relative rate of developmental failure (gray circles). Previously published constructs are included for comparison and marked by asterisks. For numbers and detailed statistical analysis see Tables S1 and S4.

(D) Deconvolved fluorescence image showing LAT-1::GFP expression at the plasma membrane of pharyngeal muscle cells (arrowheads), neurons in the nerve ring and the pharyngeal nervous system (arrows).

(E) A ΔHRM variant exhibits an expression pattern indistinguishable from the control fusion protein in (D). Scale bars represent 10 μm. See also Figures S2 and S3.