Skip to main content
. 2013 Sep 19;4:238. doi: 10.3389/fphys.2013.00238

Figure 5.

Figure 5

Effect of siRNA-mediated knockdown of canine SNAP-23 in MDCK cells. The effect of transfection of MDCK cells with scrambled siRNA (siRNA scr) or siRNA directed against SNAP-23 on spontaneous [Ca2+]i events; representative original traces shown in (A) and (B) shows the summarized data as number of events s−1 as mean ± s.e.m. (Con: n = 12, siRNA SNAP-23: n = 9, siRNA scr: n = 8). (C) The effect of knocking down canine SNAP-23 on the ability of MDCK cells to respond to ATP (100 μM, Con: n = 12, siRNA SNAP-23: n = 9, siRNA scr: n = 8). Please note that preparations, which did not respond to extracellular ATP were not included in the analysis. (D,E) Confirmation of siRNA-mediated knockdown of SNAP-23 by qPCR (D) and immunoblotting (E). The arrow indicates the reduced protein expression of SNAP-23 (~25 kDa, expected size ~23 kDa) in the lysate of cells transfected with siRNA directed against SNAP-23 (left). The control blot incubated with primary antibody, which had been pre-incubated with a SNAP-23 peptide is shown to the right. Con: n = 4, siRNA SNAP-23: n = 4, siRNA scr: n = 3. All experiments were performed at 48 h after transfection of the MDCK cells. The asterisks indicate statistical significance.