Figure 1.

P2Y2 receptor was significantly expressed in prostate cancer cells. (A) mRNA levels of P2Y receptor subtypes were detected by real-time PCR in 2B4, 1E8 and DU-145 cells. The expressions of all P2Y receptor subtypes were normalised by β-actin, and mRNA expression of P2Y1 receptor was defined as 1. (B) Protein level of P2Y2 receptor was measured using western blot analysis in prostate cancer cell lines. Results were demonstrated by histograms to quantify the expression levels. Data were presented as mean±s.d. (vertical bars). Three independent experiments were performed.