Figure 2.

PCCL-loaded TAPCells immunotherapy is able to induce specific immune response in CRPC patients. (A) PBMCs obtained before (filled circles) or at the end of treatment (clear circles) were co-cultured with DCs loaded with PCCL and in vitro immune response was evaluated by IFN-γ ELISPOT assay. Graphics are representative of four patients. (B) DTH test against tumour lysate. Thirty days after the fourth immunisation, patients were inoculated subcutaneously with PCCL, CCH, KLH or saline solution (Ctrl). Skin inflammatory reaction was evaluated 48 h later. Pictures are representative of two patients. Experiments were performed in triplicate (*P<0.05, **P<0.01, ANOVA).