Figure 7.

L-type Ca²⁺-blockade prevents CREB phosphorylation in differentiating NPCs. NPCs were cultivated either under IBMX (A) or IBMX with nifedipine (B). After 1 and 7 days, cultures were fixed and stained concomitantly for MAP2ab and phosphorylated CREB. pCREB-signal intensity was measured by confocal microscopy. In (C), pCREB signal at the beginning and end of differentiation in cultures with IBMX and IBMX+nifedipine. In (D), the pCREB/DAPI-signal ratio was calculated to correct for different cell densities. In both measurements, nifedipine prevented pCREB phosphorylation during differentiation. Scale bars 150 μM.