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Simulation of maximum frequency of minor variant to be eliminated by PyroNoise
| IFC mutations | OFC mutations | ||||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 1 |
| 19% | 2% | 0.30% | 0.04% | 0 | 0 |
Note: Flowgrams were simulated based on an amplicon of 339 bp. IFC mutations represent mutations that do not change the number of flow cycles. OFC mutations will change the number of flow cycles.
