Figure 2.

Deletion of SIT4 or KTI12 confers resistance to excess dihydroceramide. (a) Ceramide growth tests in Sit4 associated protein (SAP) mutants. (b) Ceramide growth tests in Elongator-associated mutants. Yeast cultures were diluted to 5 × 10³ cells/mL in YPD with the addition of either 15 μM C-2 dihydroceramide or an equal volume of ethanol. Cells were grown until the untreated culture reached exponential phase and the OD₆₀₀ of all cultures was determined. Growth in 15 μM dihydroceramide is expressed as a percentage of untreated growth. Raw OD₆₀₀ values are given in Supplementary Table 1. A minimum of three replicates are shown and error bars represent the standard deviation above and below the mean. A one-way ANOVA with a Bonferroni post-test was used to determine if mutants showed a significant difference in growth compared to the wild type (CY4029) (**P < 0.01, ***P < 0.001, and ****P < 0.0001).