(c)  

	PCR product	Forward PCR primer, F1	Reverse PCR primer, R1	Sequencing primer, S1
Length, bp	109	18	19	18
Position, 5′-3′		3-20	111-93	3-20
Warnings
Tm, °C		60.2	60.9	53.1
% GC	33.0	50.0	36.8	50.0
Sequence to analyze	YGAATAGGAATAGTTTYGGTTTATAGTTTTTAGYGTGAGYGAYGTAGAAGAYGGGTGATTTTTGTATTTTTATTTGA