(c)  

	PCR product	Forward PCR primer, F1	Reverse PCR primer, R1	Sequencing primer, S1
Length, bp	127	24	19	19
Position, 5′-3′		105-128	231-213	118-136
Warnings
Tm, °C		61.9	59.3	50.8
% GC	37.0	29.2	31.6	47.4
Sequence to analyze	GYGTAGGTTATTGTGTGYGYGTATYGTGYGYGAGTYGAAGTAGGGYGAGGTATTGTTTTATTTGGGAAG